Time-Resolved Fluorescence Spectroscopy of the Retinal Pigment Epithelium: Age-Related Studies
- Lipofuscin granules contain fluorophores which emit yellow-orange light upon UV illumination.
- An Ar-ion or a Kr-ion laser working in mode-locking (M-L) regime are used as the principal excitation source. The two lasers can be tuned to all their emission source. The two lasers can be tuned to all their emission lines (except for the 488 nm line of the Ar-ion laser), thus providing excitation pulses at wavelengths ranging from 364 to 700 nm at intervals of = 50 nm.
- Melanin samples exhibited fluorescence spectra peaking in the blue (440 nm, corresponding excitation peak at 350 nm.
- Lipofuscin samples exhibited a broad emission peak around 590-600 nm, with a corresponding almost flat excitation spectrum from 350 to 500 nm. A secondary peak around 650 nm appeared in samples from older age groups.
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